Si vous constatez des erreurs, veuillez contacter wes.moerman@ualberta.ca.
* Temps converti en fonction d’une piste surdimensionnée, sous-dimensionnée ou avec virages en angle.
^ Couru sur une distance qui ne figure pas au programme de U Sports.
Critères automatiques de U Sports : 54.01
| Rang | Athlète | Perf. | YOE | Compétition | Plc | Date | |
| 1 | Paige Willems | SASK | 55.71 | 3 | Penn State National Open | 8 | 31 jan |
| 2 | Elisabeth McDowell-Mitchell | VICT | 57.04 | 1 | 2025 Husky Classic | 8 | 14 fév |
| 3 | Adriana Koes | MANI | 58.91 | 1 | NDSU Bison Open | 6 | 7 fév |
| 4 | Juliana Crocker | MANI | 58.98 | 1 | NDSU Bison Open | 7 | 7 fév |
| 5 | Zoe Nonato | MANI | 59.46 | 3 | WOA Winter Open | 1 | 17 jan |
| 6 | Morgan Kehl | MANI | 59.74 | 1 | SDSU Indoor Classic | 25 | 14 fév |
| 7 | Reese Drake | SASK | 1:00.23 | 2 | Knights of Columbus Indoor Games | 24 jan | |
| 8 | Livia Blechinger | SASK | 1:00.43 | 2 | Knights of Columbus Indoor Games | 24 jan | |
| 9 | Abigail Peace | SASK | 1:01.97 | 1 | Knights of Columbus Indoor Games | 24 jan | |
| 10 | Emerson Pekush | SASK | 1:02.27 | 1 | Knights of Columbus Indoor Games | 24 jan | |
| 11 | Simone Crevier | MANI | 1:02.34 | 4 | Athletics Manitoba Last Chance Meet | 1 | 13 déc |
| 12 | Megan Hausermann | SASK | 1:03.13 | 1 | Knights of Columbus Indoor Games | 24 jan | |
| 13 | Julia Egert | SASK | 1:03.81 | 1 | Knights of Columbus Indoor Games | 24 jan | |
| 14 | Alexandra Davis | MANI | 1:06.52 | 2 | WOA Winter Open | 4 | 17 jan |
| 15 | Annika Yuzdepski | SASK | 1:07.84 | 1 | Knights of Columbus Indoor Games | 24 jan |
