If you notice any errors, please contact wes.moerman@ualberta.ca.
* Converted time based on oversize/undersize/banked track.
^ Run at a non-USports distance.
U Sports Auto Standard: 54.01
| Rk | Athlete | Perf. | YOE | Meet | Plc | Date | |
| 1 | Paige Willems | SASK | 55.71 | 3 | Penn State National Open | 8 | Jan 31st |
| 2 | Elisabeth McDowell-Mitchell | VICT | 57.04 | 1 | 2025 Husky Classic | 8 | Feb 14th |
| 3 | Adriana Koes | MANI | 58.91 | 1 | NDSU Bison Open | 6 | Feb 7th |
| 4 | Juliana Crocker | MANI | 58.98 | 1 | NDSU Bison Open | 7 | Feb 7th |
| 5 | Zoe Nonato | MANI | 59.46 | 3 | WOA Winter Open | 1 | Jan 17th |
| 6 | Morgan Kehl | MANI | 59.74 | 1 | SDSU Indoor Classic | 25 | Feb 14th |
| 7 | Reese Drake | SASK | 1:00.23 | 2 | Knights of Columbus Indoor Games | Jan 24th | |
| 8 | Livia Blechinger | SASK | 1:00.43 | 2 | Knights of Columbus Indoor Games | Jan 24th | |
| 9 | Abigail Peace | SASK | 1:01.97 | 1 | Knights of Columbus Indoor Games | Jan 24th | |
| 10 | Emerson Pekush | SASK | 1:02.27 | 1 | Knights of Columbus Indoor Games | Jan 24th | |
| 11 | Simone Crevier | MANI | 1:02.34 | 4 | Athletics Manitoba Last Chance Meet | 1 | Dec 13th |
| 12 | Megan Hausermann | SASK | 1:03.13 | 1 | Knights of Columbus Indoor Games | Jan 24th | |
| 13 | Julia Egert | SASK | 1:03.81 | 1 | Knights of Columbus Indoor Games | Jan 24th | |
| 14 | Alexandra Davis | MANI | 1:06.52 | 2 | WOA Winter Open | 4 | Jan 17th |
| 15 | Annika Yuzdepski | SASK | 1:07.84 | 1 | Knights of Columbus Indoor Games | Jan 24th |
